A Genetically Engineered Bivalent Vaccine Coexpressing a Molecular Adjuvant against Classical Swine Fever and Porcine Epidemic Diarrhea
نویسندگان
چکیده
Classical swine fever (CSF) and porcine epidemic diarrhea (PED) are highly contagious viral diseases that pose a significant threat to piglets cause substantial economic losses in the global industry. Therefore, development of bivalent vaccine capable targeting both CSF PED simultaneously is crucial. In this study, we genetically engineered recombinant classical virus (rCSFV) expressing antigenic domains (PEDV) based on modified infectious cDNA clone strain C-strain. The S1N COE PEDV were inserted into C-strain harboring mutated 136th residue Npro substituted 3′UTR generate chimeric vC/SM3′UTRN-S1NCOE. To improve efficacy vaccine, introduced tissue plasminogen activator signal (tPAs) CARD domain signaling molecule VISA vC/SM3′UTRN-S1NCOE obtain vC/SM3′UTRN-tPAsS1NCOE vC/SM3′UTRN-CARD/tPAsS1NCOE, respectively. We characterized three candidates vitro investigated their immune responses rabbits pigs. NproD136N mutant exhibited normal autoprotease activity mitigated inhibition IFN-β induction. introduction tPAs led secretory expression S1NCOE protein upregulated induction infected cells. Immunization with CSFVs resulted significantly increased PEDV-specific antibody production, coexpression IFN-γ level serum vaccinated animals. Notably, vaccination vC/SM3′UTRN-CARD/tPAsS1NCOE conferred protection against virulent CSFV challenge Collectively, these findings demonstrate promising candidate infections.
منابع مشابه
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ژورنال
عنوان ژورنال: International Journal of Molecular Sciences
سال: 2023
ISSN: ['1661-6596', '1422-0067']
DOI: https://doi.org/10.3390/ijms241511954